ResourceLife Sciences
Avoiding Cross-Contamination When Using the Harris Uni-Core Punch Tool in Thermo Scientific Direct PCR Protocols
16 Dec 2013Our Direct PCR approach saves time and cost by allowing amplification of DNA directly from various source materials. With starting materials such as plant leaves, mouse ears or filter papers, the easiest way to produce samples of small and uniform size is to use the Harris Uni-Core Punch Tool and Harris Cutting Mat Technology. We found that 2% sodium hypochlorite efficiently prevents cross-contamination of the samples from the tools.
Tags
PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Contamination PreventionContamination prevention aims to control and minimize the risk of contamination during experiments. Biological safety cabinets and sterilization equipment are examples of technologies used to prevent contamination.Direct Sampling