ResourceLife Sciences
Automation of the BioLabs NEBNext Ultra II Directional RNA Library Preparation Kit for Illumina
23 Jan 2023Laboratories are looking for reproducible next generation sequencing (NGS) sample preparation methods that limit the potential for in-process sample degradation or error. This application note details an automated process for the New England Biolabs NEBNext Ultra II Directional RNA Library Preparation Kit for Illumina that offers the laboratory optional settings to optimize a demonstrated application that will process between 4 and 24 samples from start to finish.
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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NGS AutomationNGS automation streamlines next-generation sequencing workflows, increasing throughput, accuracy, and reproducibility. Automated solutions enhance productivity in genomic research, clinical diagnostics, and personalized medicine. Browse peer-reviewed tools to compare products, read reviews, and get pricing.