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Automation of NGS Sample Size Selection Using SPRIselect on the Biomek 4000 Laboratory Automation Workstation

Beckman Coulter Diagnostics
26 Feb 2015

To streamline the NGS sample preparation process, Beckman Coulter launched the SPRIselect Reagent Kit to allow NGS sample size selection to be automated on Biomek liquid handling platforms for high throughput and high efficiency sample preparations. The data presented here demonstrates the automation of the SPRIselect Kit on the Biomek 4000 Workstation. The demonstrated method will prepare 96 samples in 96-well plate format for small to medium throughput laboratories.

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Biomek® 4000 Laboratory Automation Workstation

Beckman Coulter Life Sciences

Biomek Liquid Handlers enable you can spend less time on manual process and more time on powerful discoveries. Beckman Coulter Life Sciences is a leader when it comes to providing solutions and support that cover the full spectrum of the NGS sample preparation workflow with reagents and automation. Throughput options allow you to choose the right solution for your laboratory.

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Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.NGS

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Automation of NGS Sample Size Selection Using SPRIselect on the Biomek 4000 Laboratory Automation Workstation