Automation of a Differential Digestion Protocol for Processing Sexual Assault Kits Using the VERSA Automated Liquid Handling Workstation

20 Oct 2014

Separation of sperm cell DNA from epithelial cell DNA in processing sexual assault kits remains a time consuming, manual task contributing to the backlog of evidence processing. Rapid and accurate sample processing is of high importance to ensure DNA evidence is presented properly for potential conviction. In this application note, an automated differential digestion protocol was developed and adapted for use on the VERSA 1100 automated liquid handling workstation. The automated protocol gave DNA yields of similar concentration to the conventional manual protocol resulting in similar short tandem repeat (STR) typing profiles.

Links

Tags

Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Liquid Handling WorkstationLiquid handling workstations automate the process of sample preparation, improving accuracy and efficiency. These systems are essential for high-throughput applications in biological, chemical, and pharmaceutical research, reducing human error and increasing reproducibility. Workstations come equipped with robotic arms, pipetting tools, and other accessories to streamline lab workflows. Explore liquid handling workstations in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.
Automation of a Differential Digestion Protocol for Processing Sexual Assault Kits Using the VERSA Automated Liquid Handling Workstation