ResourceLife Sciences
Automating a Linear Density Gradient for Purification of a Protein:Ligand Complex
5 Jul 2015Proteins have variety of cellular functions, structures and mechanisms of action, routinely binding other biomolecules, or ligands, in order to complete a task. This application note describes the use of the Biomek 4000 Workstation in a simplified method for gradient preparations. To eliminate user variability, Beckman Coulter’s Biomek 4000 Workstation provides consistent and reproducible results in layering discontinuous density gradients.
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Biomek® 4000 Laboratory Automation Workstation
Beckman Coulter Life SciencesBiomek Liquid Handlers enable you can spend less time on manual process and more time on powerful discoveries. Beckman Coulter Life Sciences is a leader when it comes to providing solutions and support that cover the full spectrum of the NGS sample preparation workflow with reagents and automation. Throughput options allow you to choose the right solution for your laboratory.
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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.Liquid Handling WorkstationLiquid handling workstations automate the process of sample preparation, improving accuracy and efficiency. These systems are essential for high-throughput applications in biological, chemical, and pharmaceutical research, reducing human error and increasing reproducibility. Workstations come equipped with robotic arms, pipetting tools, and other accessories to streamline lab workflows. Explore liquid handling workstations in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Clinical AutomationClinical laboratory automation refers to the use of automated systems in clinical laboratories to increase efficiency, reduce human error, and improve patient care. From sample analysis to total laboratory automation, this plays a critical role in modern diagnostics and laboratory workflows. Explore clinical automation solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Protein PurificationLigands