Automated viral plaque assay workflow using the Cytation Cell Imaging Multimode Reader

15 Mar 2023

Plaque assays remain the standard method for determining viral titers for lysogenic viruses. The assay relies on determining the number of plaque-forming units (PFU) created in a monolayer of virus-infected cells. In this application note, Agilent presents an assay that provides an accurate and efficient method for determining plaque counts and calculating viral titers in a range of microplate densities, including 6-, 24-, 48-, and 96-well formats.

Agilent BioTek Gen5 Software for Detection

Agilent Technologies

Agilent BioTek Gen5 software for Agilent BioTek multimode and single-mode microplate readers is an integrated tool for endpoint, kinetic, spectral scanning, and well area scanning. It controls all the functions of the plate reader and has powerful data analysis capabilities for a broad range of applications. Gen5 microplate reader software is also used to integrate BioTek plate readers to BioTek BioStack and other automated systems.

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Automated viral plaque assay workflow using the Cytation Cell Imaging Multimode Reader