Automated TRANSIL<sup>®</sup> HSA Binding Kit on CyBi-Well® Vario

13 Aug 2014

This application note describes the HTS set-up of the ADME/Tox determination of human serum albumin binding using TRANSIL® HSA Binding Kit and the CyBi-Well® Vario with the 384/25 µL head.

CyBio Well Vario

Analytik Jena GmbH+Co. KG

The CyBio Well Vario is CyBio´s automated, simultaneous pipetting platform which can be equipped with 11 interchangeable pipetting heads to offer highest pipetting precision and flexibility in a compact design. A large variety of disposable tips, highly precise ceramic tips or glass capillaries allow liquid transfers in a broad volume range of 4 orders of magnitude (25 nl up to 250 µl) on one pipetting system. 14 different capillary magazines are available for CyBio´s Capillary Head, which is especially designed for precise low-volume compound transfers (25—1000 nl). The CyBio Well vario is compatible with many specific accessories and can be adapted to every screening environment to create powerful liquid handling platforms for today and tomorrow. Optional stacker or dispenser integration together with the involvement of versatile peripheral devices can be easily implemented. Challenging liquid handling applications like working in high density microplate formats or in HTS environments can be easily achieved. CyBio´s outstanding customer service and application support is a well appreciated inherent part of every CyBio Well vario system. CyBio Well Vario Features: Extremely flexible through enormous volume spectrum Highest precision in the whole volume range Unique pipetting technology prevents cross contamination Easy application set-up using CyBio Composer software Saves reagent costs with accurate low volume pipetting

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MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ADMEHuman SerumHuman serum is found in blood plasma. The protein is used in a variety of applications including tissue engineering, transplantation and cell therapy.
Automated TRANSIL<sup>®</sup> HSA Binding Kit on CyBi-Well® Vario