ResourceLife Sciences
Automated Targeted Locus Amplification (TLA) Technology for Targeted Complete Gene Sequencing Using the JANUS NGS Express Workstation
18 Nov 2014TLA sample preparation, like all NGS applications, necessitates robust library preparation to yield high-quality DNA samples. This application note describes the automation of TLA Technology using the JANUS NGS Express Workstation. The method is designed to increase throughput and walkaway time, while also eliminating inter-operator variability in sample preparation performance as well as errors in sample tracking.
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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.