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Automated Solid Phase Extraction of 14 Explosives in Tap Water Based on U.S. EPA Method 8330 Using Hydrophilic Reversed-Phase Cartridge Followed by HPLC with UV Detection

28 Aug 2014

This application note investigates the concentration of 14 Explosives in fortified tap water by automated SPE using the Dionex AutoTrace 280 and a new polymeric sorbent SolEx SPE HRPHS cartridge.

Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction Instrument

Thermo Fisher Scientific

The Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction (SPE) instrument is a powerful, high-throughput workstation dedicated specifically for automating SPE. The Dionex AutoTrace 280 instrument automates the cartridge or disk conditioning, sample loading, rinsing, and eluting steps for large volume aqueous solution extractions (20 mL to 20 L).The unit can process up to six samples in parallel, automatically conditioning, washing, and eluting SPE cartridges or disks with a choice of up to five reagents. The unit also segregates aqueous and solvent waste using separate waste lines for efficient, cost effective waste handling.AutoTrace 280 Solid-Phase Extraction Instrument Features: Use SPE technology for liquid-liquid extraction Automated sample loading of liquids onto SPE cartridges Automated eluting of SPE cartridges with organic solvent Closed systems with a fan to vent solvent vapors Positive pressure loading and elution of samples and solvents Benefits: Reduced solvent usage and elimination of glassware Does not require technician involvement to maintain a reservoir of liquid in the SPE cartridge Does not require technician involvement to control the flow of organics solvent through the SPE cartridge No hood required Provides constant flow of liquids through SPE cartridges

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Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solid Phase Extraction (SPE)Solid phase extraction (SPE) is a sample preparation technique used to separate and concentrate analytes from complex mixtures. It is widely used in environmental, pharmaceutical, and clinical analysis. SPE provides higher sensitivity and selectivity compared to traditional liquid-liquid extraction methods, making it essential for accurate and reproducible results. Explore SPE tools and systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Environmental ContaminationContaminants in the environment are harmful chemicals that are present in the air, land and water. These include pesticides, pharmaceuticals, persistent organic pollutants (POPs) and many more.
Automated Solid Phase Extraction of 14 Explosives in Tap Water Based on U.S. EPA Method 8330 Using Hydrophilic Reversed-Phase Cartridge Followed by HPLC with UV Detection