ResourceLife Sciences
Automated Purification of DNA from Urine for Sensitive PCR of Chlamydia Trachomatis Amplicons
27 Nov 2013Development of sensitive and reproducible assays for clinical and life science research of C. trachomatis is essential to monitor and help prevent further spread of this pathogen. DNA was purified from 130 urine research samples using a fully automated protocol on the BioRobot® M48 workstation together with the MagAttract® DNA Mini M48 Kit. Purified DNA performed well in a real-time PCR assay being developed for the amplification of Chlamydia trachomatis DNA. Comparison of this easy-to-use procedure with a commercial C. trachomatis assay and manual DNA purification showed identical sensitivity.
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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ChlamydiaDNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.Sexual Health