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Automated Library Preparation using NEBNext Ultra DNA Kit on ACSIA NGS<sup>LibPrep</sup>Edition

PrimaDiag
27 Jun 2016

This application note demonstrates the efficiency of library preparation using NEBNext Ultra DNA Lib Prep kit with ACSIA NGSLibPrepEdition in comparison to a manual method. Performing NGS requires time consuming library preparation, which can be drastically reduced through automation. To meet this demand, PrimaDiag developed the NGSLibPrep system based on ACSIA platform using the latest technologies, which allow for the handling of magnetic beads, control of the temperature with 0.1°C of accuracy, and shaking of samples from 200 to 3000 rpm.

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ACSIA NGSLibPrep Edition

PrimaDiag

ACSIA NGSLibPrep Edition, the solution to automate the libraries preparation for next-generation sequencing. In view of increasing your productivity, avoiding contamination and improving your work efficiency, PrimaDiag created ACSIA NGSLibPrep Edition. It uses our software PrimaControlleur®II and has been designed to allow you to effectively perform your libraries preparation.ACSIA NGSLibPrep Edition allows you to perform 96 samples without human intervention libraries. To do so, it operates from just after the fragmentation up through the final PCR amplification. It is in this last step at the end of the process that a sufficient quantity of interesting fragments is obtained. Moreover, with our software and its innovative SMT technology, it ensures lower process costs by reducing the quantity of tips used during your manipulations. ACSIA NGSLibPrep Edition minimizes the use of your reagents and consumables during your processes and prevents waste. It also ensures minimal sample loss during treatment. At the end of your processes the yield with ACSIA NGSLibPrep Edition is increased by 20% over manual treatment and process costs are and highly reduced.To perform the libraries preparation, ACSIA NGSLibPrep Edition is available with 16 SBS positions integrating our patented Magnetic Work Unit and our heatingShaker 3000. This configuration is provided with a 250µL 1-channel pipetting head and a 250µL 8-channel pipetting head. Its’ PrimaController®II software offers both a realistic and interactive view as well as real-time assistance to increase your efficiency by letting you focus on your own tasks.Features: Magnetic Work Unit based on our patented APIM technology (Attraction Point by Induced Magnetization) Pipetting heads are interchangeable within seconds High efficiency automated platform managed by innovative, smart software Easy and User-Friendly programming Pre-programmed protocols for exome capture Add-in our protective enclosure for better safety during your processes One of the market's best quality/price values

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Robotic InstrumentsRobotic instruments can be used for high-throughput automation of many lab processes. Such processes use instruments for assays like cell based assays and ELISA, for sample preparation like shakers, centrifuges and incubators, and for analysis such as sequencing and western blot analyzers. Useful features of robotic instruments include speed, reproducibility, barcode readers, software and automation.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Plate ProcessingAutomated plate processing is used in microplate technologies for high-throughput, speed and efficiency. Plate processing systems include microplate handlers for moving plates, heat sealers, stackers and carousels for storage, retrieval and delivery, microplate washers and piercers. Useful features of plate processors include walk-away automation, compatibility and easy integration with microplate systems.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Plate ProcessingHigh ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.

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Automated Library Preparation using NEBNext Ultra DNA Kit on ACSIA NGS<sup>LibPrep</sup>Edition