SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

Automated Imaging-Based Technique for Monitoring of Ca2+ and DAG Biosensors Enables Detailed Characterization of GPCR Kinetics

Agilent Technologies
8 Nov 2018

This poster describes an automated imaging-based approach to characterize the IP3 /DAG signaling pathway using multiplexed R-GECO Ca2+ and DAG fluorescent biosensors from Montana Molecular. This method produces detailed kinetic profiles of Ca2+ flux and DAG levels following activation of Gq-coupled receptors within the same population of cells. Background subtraction and image analysis tools enable detection of changes in Ca2+ and DAG levels that are more sensitive than techniques relying on bulk fluorescence measurements. Additionally, this method enables single-cell analysis for detailed characterization of response dynamics and subcellular effects.

Related products

Request Quote for All Products

Links

Agilent TechnologiesCompany website

Tags

BiosensorsBiosensors are devices used to detect an analyte using biological molecules specific to the analyte coupled to a detector. Biosensor instruments may be photometric, typically using surface plasma resonance (SPR), electrochemical or QCM (quartz crystal microbalance) biosensors. Biosensors should be selective, portable, robust and sensitive and have a fast response time. Systems may be manual or automated and usually have associated software.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.ImagingImaging techniques are essential for obtaining visual representations of samples to understand structures, processes, and function in biological, chemical, and physical research. These tools range from traditional light microscopy to advanced imaging modalities like MRI and electron microscopy, providing researchers with valuable data for diagnostics, drug discovery, and material analysis. Explore imaging solutions in our peer-reviewed product directory to compare products, check reviews, and get pricing directly from manufacturers.Drug DiscoveryDrug discovery is the process of identifying potential new medications, involving stages such as target identification, compound screening, and preclinical development. It relies on cutting-edge technologies like high-throughput screening, artificial intelligence, and molecular modeling to accelerate the identification of drug candidates. Drug discovery plays a pivotal role in developing new therapies for diseases ranging from cancer to rare genetic disorders. Browse our peer-reviewed product directory to find the latest drug discovery technologies, compare options, check customer feedback, and get pricing directly from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

Related resources