Automated image analysis reduces user-to-user variability in flow cytometry gating strategies

The need for high-throughput sample acquisition and automation in flow cytometry creates demand for new methods for analytical standardization and for reducing sources of variability. When performed on the Attune CytPix Flow Cytometer, automated image analysis combined with conventional flow cytometry provides a robust quantitative method for standardizing flow cytometry data analysis. In this application note, Thermo Fisher Scientific demonstrates that automated image-assisted analysis enables more accurate gating of true singlet populations and exclusion of unwanted events.