Automated IgG Purification Using PureProteome™ Magnetic Beads with the KingFisher®Particle Processor
24 Sept 2013This application note evaluates the effectiveness of PureProteome™ Protein G Magnetic Beads for IgG purification in a fully automated purification process using the KingFisher® Duo particle processor. To obtain the best results, parameters such as mixing speed, bead collection time and duration of binding and washing steps were optimized for use with PureProteome™ Protein G Magnetic Beads.
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PureProteome™ Protein G Magnetic Bead System
MerckThe PureProteome Protein G Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability. PureProteome Protein G magnetic beads are a powerful system to isolate antibodies faster, easier, and with more reproducibility then ever before. PureProteome Protein G magnetic beads purify your sample with the highest binding capacity of any magnetic beads available. You can achieve reproducible results in both immunoprecipitation and serum depletion assays.PureProteome™ Protein G Magnetic Bead System Features: High capacity: Advanced chemistry combined with high surface area provides more binding sites for proteins. Consistent results with no sample loss: Particles visible as they adhere to side of tube for quick and easy aspiration and complete buffer removal. Fast processing time: Beads are immobilized in seconds. Increased kinetics of bead-protein binding enables shorter incubations. Economical: Significantly more affordable then competitive magnetic beads.