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Automated, High-Throughput LC-MS/MS Workflow for the Analysis of 25-Hydroxyvitamin D2/3 and 3-Epi-25-Hydroxyvitamin D3

10 Mar 2015

In this application note, an automated, high-throughput LC-MS/MS workflow was developed for the analysis of 25-hydroxyvitamin D2/3 and 3-epi-25-hydroxyvitamin D3 in human serum for research laboratories.

Thermo Scientific Versette

Thermo Fisher Scientific

The Thermo Scientific™ Versette is a compact automated liquid handler that offers 96- or 384-channel pipetting heads and user friendly programming options. Its reliable performance and compact size make it a flexible and convenient liquid handling solution for many kinds of laboratories. Versette is designed to perform a variety of liquid handling tasks for a wide range of applications including high-throughput immunoaffinity sample preparation with Mass Spectrometric Immunoassay (MSIA*) pipette tips. It also supports 96/384 plate replication, plate stamping and serial dilution procedures.Key Features: 96-and 384-channel pipetting heads Handle a total volume range of 0.5-300 µl All pipetting heads include RFID tags for self-identify, and utilize Thermo Scientific D.A.R.T.s tips Compact Size The six position stage uses a unique dual-level design to minimise space requirements Fits on standard benches and biological hoods User-friendly programming options: Simple pipetting procedures can be created using visual on-board graphical display For more complex protocols and advanced editing Thermo Scientific ControlMate software is available

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.Vitamin D
Automated, High-Throughput LC-MS/MS Workflow for the Analysis of 25-Hydroxyvitamin D2/3 and 3-Epi-25-Hydroxyvitamin D3