ResourceLife Sciences
Automated hemocytometer-based live/dead Cell Counting using hase contrast and color brightfield imaging
13 Jul 2020In this application note, BioTek demonstrates how the hemocytometer cell counting process can be automated using a Cell Imaging Multi-Mode Reader equipped with contrast enhancement technologies. For total cell counts, the Cell Imager uses automated phase-contrast microscopy to count the total number of cells in the field of view which is about 2.9 times larger than the total ruled grid area. This serves to improve the counting statistics relative to counting manually. Furthermore, cell viability can also be determined through the use of automated color imaging of tryptan blue-stained cells in the same field of view.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Colony CountingCell SortingCell sorting isolates specific cell populations based on physical or molecular characteristics, essential in immunology, cancer research, and regenerative medicine. Techniques like flow cytometry improve accuracy and speed. Compare cell sorting instruments and solutions in our product directory.Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.