ResourceForensics
Automated Extraction of Isomeric Cortisone and Prednisolone from Plasma using Novum™ Simplified Liquid Extraction (SLE) and Analysis by a Kinetex® Core-Shell Biphenyl HPLC/UHPLC Column
5 Feb 2015Corticosteroids exhibit anti-inflammatory properties and are widely abused within the sports industry. Chromatographic separation is essential for detection and confirmation of corticosteroids because cortisone and prednisolone are isomeric compounds. A simple automated extraction method using Phenomenex’s Novum Simplified Liquid Extraction (SLE) is employed to extract corticosteroids from plasma samples. A Kinetex 2.6 μm Biphenyl HPLC/UHPLC column is used to successfully separate these two compounds.
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Novum Simplified Liquid Extraction (SLE)
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.SteroidsPerformance-Enhancing Drugs