ResourceLife Sciences
Automated Cell Counting via Flow Cytometry
7 Feb 2019Cell counting is an often overlooked, yet critical basis for experiments and production processes. Methodology for cell counting spans multiple technologies that include fluidic-based systems or immobilized slide-based techniques. These methods range from manual to fully automated counting, depending on the device. While there are numerous options available, consistency and reproducibility often fall short. Especially manual counting techniques can be extremely variable with coefficients of variation (CV) as high as 27%.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.CytometryCytometry is a broad term for the quantitative analysis of cells and cell systems. The most popular methods to study cytometry are flow cytometry and image cytometry.
