Automated 3D spheroid culture media exchange using a novel MultiFlo™ FX accessory

Culturing cells in 3D provides a more in vivo-like environment, allowing cells to maintain high viability when cultured for extended time periods. To maintain the highest levels of viability within untreated cells and ensure that observed effects are solely from treatment, media exchanges and re-dosing are necessary throughout the experiment, particularly in vitro tests lasting weeks. Through incorporation of the AMX™ Automated Media Exchange module on the MultiFlo™ FX Multi-Mode Dispenser, risk of accidental spheroid removal from wells is eliminated. Spent media is automatically removed and replaced with fresh media only, or fresh media containing treatment concentrations.