SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

Assuring Optimal Promega Assays Development by Design of Experiment Workflow and Mantis Dispenser

Promega Corp.

Assuring Optimal Promega Assays Development by Design of Experiment Workflow and Mantis Dispenser

29 Dec 2015

To facilitate assay development a Design of Experiment approach can be employed during assay development. Design of Experiments (DoE) offers a systematic way of simultaneously evaluating multiple assay parameters and analyzing the resulting process outputs to optimize assay conditions with a minimum number of experiments. One of the limitations in taking full advantage of the benefits offered by DoE is the complexity of experimental set up. This poster demonstrates use of the Mantis instrument from Formulatrix, simplifying DoE setup by providing the unique capabilities of dispensing small volumes of multiple assay components with minimal dead volume into randomly designated wells. Flexibility in the dispensing capabilities and user friendly programming makes this instrument well suited for DoE workflow.

Links

Promega Corp.Company website

Tags

MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.Reagent DispenserMethod DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.

Related resources

Assuring Optimal Promega Assays Development by Design of Experiment Workflow and Mantis Dispenser