Assessing T cell bioenergetic poise and spare respiratory capacity using extracellular flux analysis

15 Mar 2023

The Agilent Seahorse XF T Cell Metabolic Profiling kit is a robust solution for the complete real-time assessment of T cell metabolism. In this application note, learn more about using the Agilent kit to assess the impact of the medium composition during T cell expansion following the XF T Cell Persistence Assay workflow. This workflow allows for the evaluation of glycolysis and mitochondrial activity and capacity simultaneously, providing a comprehensive metabolic profile of T cells that can be incorporated into monitoring and improving the design and development of T cell therapies.

Seahorse XF Pro Analyzer

Agilent Technologies

Measure mitochondrial respiration, glycolysis, and ATP production rates in live cells in a 96-well forma t, providing a system-level view of cellular energy metabolism and mitochondrial health. It combines enhanced sensitivity and data consistency with intuitive software and advanced data analytics, enabling users to streamline assay workflow from start to finish and generate and interpret metabolic data easily. 

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GlycobiologyGlycobiology encompasses research into the biological functions, synthesis and structures of glycans, including glycoproteins, glycolipids, proteoglycans and free oligosaccharides. Mass spectrometers and their software are commonly used in the study of glycobiology, along with structure predicting tools, chromatography and NMR.Cellular BioenergeticsCell TherapyCell therapy involves using living cells to treat diseases, often by replacing damaged cells or stimulating regeneration. Stem cell therapy and CAR-T cell therapy are examples of cutting-edge treatments in regenerative medicine and cancer immunotherapy. Browse our peer-reviewed product directory to find the best cell therapy tools, compare products, check reviews, and get pricing directly from manufacturers.Glycolysis
Assessing T cell bioenergetic poise and spare respiratory capacity using extracellular flux analysis