ResourceLife Sciences
Assessing Epigenetic Enzyme Activity Using HTRF® Epigenetic Assays from Cisbio with the PHERAstar FS from BMG LABTECH
2 Apr 2017This application note describes the verification of the performance of a Cisbio HTRF® based assay using the PHERAstar FS from BMG LABTECH. This assay allows users to detect the activity of G9a, a histone methyltransferase that methylates histone H3 on lysine 9 (H3K9). G9a mediated H3K9 methylation has been shown to be involved in transcriptional repression of developmental genes which is essential for early embryogenesis. In addition G9a has been shown to have an oncogenic function based on its ability to sustain cancer cell proliferation. Therefore, G9a is an excellent example of the type of epigenetic target that is of high importance in drug development.
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PHERAstar® FSX HTS Microplate Reader
BMG LABTECHThe most sensitive microplate reader for high-throughput screening
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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Epigenetic AnalysisEpigenetic analysis products are used to study heritable phenotype changes that do not alter the DNA sequence, such as DNA modifications affecting gene activity. Determine whether genes have been methylated with DNA methylation quantification kits or methylation microarrays. Assess epigenetic regulation with histone modification kits or ChIP-on-chip microarrays. Kits are also available for bisulfite conversion and amplification of your samples, as well as epigenomic library preparation kits for next-generation sequencing (NGS). Find the best epigenetic products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SLASThe Society for Laboratory Automation and Screening (SLAS) is an organization focused on laboratory automation, high-throughput screening, and biotechnology innovation. SLAS promotes scientific advancements through conferences, publications, and industry collaborations.