ResourceLife Sciences
Assessing Enriched Murine CD4+ T Cells Differentiated Towards Effector T Helper Cell Lineages with the Scepter™ 2.0 Handheld Automated Cell Counter
19 Aug 2013T cell differentiation and its role in the immune response are integral for both innate and adaptive immunity. Differentiation of CD4+ T cells into fully functional effector T cells is characterized by both the production of signature cytokines as well as a concomitant increase in cell size. In this application note Th1, Th2, and Th17 cells were shown to expand from approximately 6 to 10 μm when compared to the progenitor CD4+ T cell type. This expansion upon differentiation was clearly, accurately, and precisely measured using the Scepter™ cell counter.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.CytokinesCytokines are signaling proteins critical for cell communication in the immune system. They regulate inflammation, immune responses, and cell growth. Cytokine research is vital for understanding disease mechanisms and developing therapies. Explore tools for cytokine detection and analysis in our directory.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.CD4 MonitoringT Cells