Assay Assembly for Miniaturized Quantitative PCR in a 384-well Format Using the Echo® Liquid Handler
18 Aug 2011The capabilities of the Echo liquid handler to dispense into 384-well qPCR plates with total reaction volumes as low as 250 nL is highlighted in this application note. Standard deviations of less than 0.25 and CVs of less than 2% are seen routinely across plates using the low volume dispensing of the Echo liquid handler. The ability of the Echo liquid handler to transfer from any well to any well simplifies assay setup. The results demonstrate the capabilities to enable assay setup with flexible plate layouts, reduced reagent volumes and increased throughput.
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Echo® 500 Series Liquid Handlers
The Echo® liquid handler revolutionizes liquid handling with acoustic energy. Sound waves eject precisely sized droplets from a source onto a microplate, slide or other surface suspended above the source. This product does not use tips, pin tools or nozzles—completely eliminating contact between the instrument and the liquid. The Echo liquid handler provides powerful benefits: Tipless transfer, resulting in dramatic cost savings Improved information about sample integrity and improved transfer quality through measurement of DMSO hydration level Elimination of the need for calibration through Dynamic Fluid Analysis™ technology, a patented Labcyte process Elimination of cross-contamination, carryover or leachates through non-contact transfer Accurate, precise movement of nanoliter-scale quantities, enabling assay miniaturization and further reducing costs Better results through direct dilution of compounds, an improvement over serial dilution methods Greatly simplified assay development and dilution processes through the flexibility to transfer any volume of liquid from any well to any other well