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Applied Robotics for Enhanced Throughput Options in Microscopy as Demonstrated by Automated Tissue Microarray (TMA) Imaging

Agilent Technologies
16 Dec 2014

Tissue microarrays (TMAs) provide a high throughput microscopy sampling format and have proven useful in many applications such as bio banking and archiving of biopsy and other tissue samples; disease diagnosis, classification and grading; quality control; and antibody optimization during assay development. In this study, TMAs were chosen as a model for demonstrating montage and stitching image optimization at high throughput using the Cytation™ 5 Cell Imaging Multimode Reader and BioStack 4 from BioTek.

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Agilent BioTek BioStack Microplate Stacker

Agilent Technologies

The Agilent BioTek BioStack microplate stacker is a compact and versatile microplate stacker, compatible with Agilent BioTek washers, dispensers, plate readers, and imaging systems. The rapid plate exchange speeds deliver increased throughput and enhanced efficiency and greater productivity.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Robotic InstrumentsRobotic instruments can be used for high-throughput automation of many lab processes. Such processes use instruments for assays like cell based assays and ELISA, for sample preparation like shakers, centrifuges and incubators, and for analysis such as sequencing and western blot analyzers. Useful features of robotic instruments include speed, reproducibility, barcode readers, software and automation.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Tissue ProcessingTissue processing involves preparing biological tissue samples for microscopic analysis, including fixation, embedding, and sectioning. This process is essential for histopathology and diagnostic applications. Explore tissue processing products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.

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Applied Robotics for Enhanced Throughput Options in Microscopy as Demonstrated by Automated Tissue Microarray (TMA) Imaging