ResourceSeparations

Applications of the New Eppendorf 5.0 mL system

14 Nov 2013

The new Eppendorf Tube 5.0 mL is a practical alternative for sample volumes in the range between 2 and 5 mL. It provides comfortable and ergonomic handling, combined with a lower risk of contamination. Compared with aliquoting the sample into several smaller tubes, considerably less work is required. Owing to its conical shape, which is akin to that of a 15 mL tube, it can be easily integrated into many existing workflows. Simultaneously, an extensive collection of accessories is now available, providing the basis for safe performance of standard applications such as pipetting, centrifugation, mixing, temperature control and storage.

Eppendorf Tubes® 5.0 mL

Eppendorf

Eppendorf Tubes are the benchmark for simple and safe sample preparation from 0.5 to 2.0 mL. For larger volumes, 15 mL and 50 mL conical tubes are available. With medium sample volumes you may face an issue: they need to be processed with large conical screw cap tubes - impractical, inconvenient and often prone to contamination. Eppendorf now offers the "missing link": the Eppendorf Tube 5.0 mL. This product fills the gap between existing tube versions and enables the simple and safe processing of sample volumes up to 5.0 mL!Eppendorf Tubes® 5.0 mL Features: Ergonomic operation - Simple, practical and ergonomic single-hand operation. Large labeling area. Lid design - Hinged lid for minimized sample evaporation during storage and incubation in a wide range of temperatures from -86 °C to 80 °C. Material - Exceptionally high-quality, transparent polypropylene, free of plasticizers, biocides or mold release agents, for reliable test results. Certified purity - Available in lot-tested and certified Eppendorf Quality, PCR clean, Sterile and Eppendorf Biopur® purity levels. Centrifugation safety - Maximum safety and stability for centrifugation up to 25,000 × g and fast and efficient protocols. Conical shape - Compatible with accessories for conical 15 mL tubes - many existing adapters and racks can be used. Sample recovery - Available in Eppendorf LoBind® material for maximum recovery of valuable samples. Applications: Easier processing of samples up to 5.0 mL Higher yields in DNA isolation, especially with samples with limited quantity of source material Convenient and safe cell culture applications Easier sample access lowers the risk of contamination Preparation of mastermixes and buffers Less storage space needed Safe cell and tissue lysis

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.CentrifugesCentrifuges are used to separate particulates suspended in a liquid via the application of a centrifugal force including cells, macromolecules, nanoparticles and precipitates. Centrifuges come in three sizes: microcentrifuges, benchtop and floor-standing centrifuges, depending on the volume and number of samples that require separation. When choosing a centrifuge, consider its maximum separation power indicated by its relative centrifugal force (RCF) value. Low force centrifuges reach a maximum RCF of 8,000 g, high-force centrifuges can reach maximum RCF values of 80,000 g. For extremely high forces, consider ultracentrifuges (up to 800,000 g). Refrigerated and vacuum centrifuges also known as concentrators are available for more specialized applications. A range of volumes are available for centrifuge tubes and microfuge tubes, depending on sample sizes. Find the best centrifuges in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample HandlingProper and careful handling of samples is important in scientific studies to ensure the integrity of specimens. This will prevent deterioration and cross contamination, and improve the accuracy of sample tracking procedures.Sample StoragePreserving clinical trial samples to the highest standards is a critical component in maximizing the value of present day and future clinical research. Specialized storage and transportation of these tissue, blood and serum samples reduces the risk of them being damaged or lost.
Applications of the New Eppendorf 5.0 mL system