ResourceLife Sciences
Application overview: The Sapphire Biomolecular Imager
19 Apr 2021In this application note, Azure Biosystems provides an application overview to the Sapphire Biomolecular Imager, a system designed to deliver outstanding, quantitative detection with NIR and RGB fluorescence, chemiluminescence, and phosphorimaging.
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Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel ImagingGel imaging captures high-resolution images of electrophoresis gels to analyze DNA, RNA, and proteins. Essential in molecular biology, this technique helps visualize results for accurate quantification and documentation. Discover top imaging systems and compare user-reviewed options in our directory.Lab EssentialsLab essentials include laboratory consumables that are vital to the operation of all laboratories.