ResourceLife Sciences
Application of the Profinity eXact™ Fusion-Tag System to Eukaryotic Expression Systems
12 Apr 2015Protein overexpression and affinity purification are used in various biological research fields and play an important role in the postgenomic era. This application note demonstrates small-scale transfection of insect Sf9 cells and mammalian HeLa cells followed by expression and purification analysis. The results indicate that the Profinity eXact system can be used in eukaryotic cells for single-step purification of tag-free proteins with low background and without compromising the obvious function of the system.
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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein Expression