Application of the Megaruptor® for Shearing of Ultra-Long DNA Fragments in the Context of Long Read Sequencing Using 3rd Generation Sequencing Technologies

Diagenode’s ultimate solution for long fragment library preparation between 2kb and 75kb– the Megaruptor® fragmentation system. A number of applications for massively parallel sequencing, also known as Next-Generation Sequencing, require libraries of long DNA segments, but few methods have been available for reliably generating fragments greater than 2 kb. The Megaruptor, which has been used by several sequencing centers worldwide to produce insert fragment libraries for Illumina, Ion Torrent, and PacBio platforms, utilizes mechanical shearing to generate libraries between 2 kb and 75 kb. The instrument’s unique feature is the “Hydropore” shearing device that eliminates clogging issues frequently encountered with single orifice shearing devices and includes a disposable element that reduces the potential for cross-contamination. A common application that requires long insert paired-end DNA libraries is mate pair library preparation, useful for de novo sequencing, detecting structural variants, and identifying rearrangements, duplications, and repeats in the genome. Features & Benefits: Achieve a tight distribution of fragment lengths, easily tuneable to between 2 kb and 75 kb Obtain high-quality libraries for Illumina®, Ion Torrent™, and PacBio® platforms Process 2 samples in series with walk-away capability Alleviate clogging issues typically encountered with single-orifice devices thanks to innovating technology Eliminate cross-contamination with disposable element Cost-effective solution