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Application of Centrifugal Vivapure Ion-Exchange Membrane Devices to the Purification and Polishing of His-Tagged Proteins for Crystallization
4 Feb 2015This study aimed to purify a bacterial enzyme to obtain crystals of sufficient quality to allow structure determination. This application note demonstrates the use of centrifugal Vivapure ion exchange membrane devices for rapid comparison of ion exchange membranes. We show that conditions identified for efficient target protein adsorption and elution also allow efficient adsorption and elution with the equivalent high binding capacity ion exchange FPLC membrane adsorbers from Sartorius, providing simple scale-up to preparative scale purification.
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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ManufacturingManufacturing is the production of merchandise for use or sale using labor and machines, tools, chemical and biological processing, or formulation.