ResourceLife Sciences
Applicability of Function Tested RealTime ready Assays for Gene Expression Analysis in Biomarker Research and Early Drug Development
26 Jun 2013Personalized health care (PHC) uses gene-based information to understand requirements for health maintenance, disease prevention, and therapy, all tailored to an individual’s genetic uniqueness. The identification and characterization of molecular biomarkers, used as indicators for a certain biological state, plays an important role in personalized healthcare. Biomarkers are therefore a central element in a drug’s lifecycle, from target identification to drug application.
Prostate-specific antigen (PSA), c-reactive protein (CRP), and HER2/neu are just a few examples of molecular biomarkers. In biomarker research, the investigation of mRNA expression profiles in the context of complex biological pathways is of great interest. Download this application note to read how RealTime ready RT-qPCR assays can be successfully applied in biomarker research using both in vivo and in vitro models and FFPE tissues.
Tags
Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :In VitroIn vitro refers to experiments conducted outside living organisms, often in controlled lab environments such as petri dishes or test tubes. In vitro models are widely used in drug testing, cell biology, and disease research. Explore in vitro research tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.In VivoFFPEFormalin-fixed paraffin-embedded (FFPE) tissues are commonly used in medical research, particularly in histology and cancer studies. The preservation process allows long-term storage of tissue samples for later analysis. Explore FFPE research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.RT qPCRReal-time quantitative PCR (RT-qPCR) is a technique used to measure gene expression and quantify DNA or RNA in real-time. It is essential in genomics, diagnostics, and biotechnology research. Explore RT-qPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.