ResourceSpectroscopy
Analyzing Cell Subsets and Heterogeneity from Live Cells
31 Jul 2018Considerable heterogeneity of individual cells exists even in the simplest cell system. Such heterogeneity is mirrored by the diversity of pharmacological responses at the cellular level, where even seemingly identical cells may respond differently to drug treatments and perturbagens. Hence, analysis at the cell-by-cell level promises additional biological insights beyond which whole population measures may deliver. In this white paper, we illustrate techniques of analyzing living cells over time, and describe new, industrial-scale analysis solutions for quantifying the phenotypic biology of cell subsets in heterogeneous cultures.
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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. 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