ResourceSeparations

Analysis of sugar alcohols and allulose using an Arc HPLC System with refractive index detection

22 Jun 2022

In this application note, an analytical method was developed to detect and quantify sugar alcohols and allulose in beverages and other low-calorie products. Arc HPLC-RI was combined with an Atlantis Premier BEH Z-HILIC analytical column, which provided an increase in polar analyte retention and offered a different selectivity when compared to other hydrophilic interaction liquid chromatography (HILIC) chemistries.

Atlantis Premier BEH Z-HILIC Column

Waters

Atlantis Premier BEH Z-HILIC Columns provide an increase in polar analyte retention and offer a different selectivity when compared to other HILIC chemistries, both powerful attributes when performing HILIC methods development.  Built using rugged ethylene bridged hybrid particles, these columns are pH stable from pH 2-10; the MaxPeak High Performance Surface hardware technology significantly reduces unwanted analyte/surface interactions that can lead to poor peak shape and losses in signal intensity.

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Arc HPLC System

Waters

Count on the Waters Arc™ HPLC System for high-efficiency separations and quality data to help meet your regulatory requirements with confidence.  The Arc HPLC system allows you to easily replicate and improve the performance of existing LC methods without compromising data quality.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HILICBeverage AnalysisBeverage analysis involves testing and evaluating the composition, quality, and safety of drinks, from alcoholic beverages to soft drinks. Techniques like chromatography and spectroscopy are commonly used to analyze flavors, nutrients, contaminants, and additives. Browse our peer-reviewed product directory to find the best beverage analysis tools, compare products, check reviews, and get pricing directly from manufacturers.
Analysis of sugar alcohols and allulose using an Arc HPLC System with refractive index detection