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Analysis of sucrose proposed in USP-NF Pharmacopeial Forum (SC1011-7F)

Showa Denko Europe GmbH
3 Mar 2023

According to the United States Pharmacopeia and the National Formulary Pharmacopeial Forum, sucrose assay and analysis of impurities (related substances) should be carried out with a HPLC method and a column filled with L19 packing material, and meet certain requirements. In this application note, Showa Denko shows that the use of a sugar analysis column, EP SC1011-7F, which confirmed the requirements were met.

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SUGAR SC1011

Showa Denko Europe GmbH

Sugar series columns using dual mode of Ligand Exchange and Size Exclusion Chromatography for specific separation of sugars. Separates mono and di-saccharides by combination of ligand exchange and size exclusion mode Counter ion: Ca2+ (divalent metal ions attached to the packing material separate saccharides from sugar alcohol as these columns retain sugar alcohol strongly.) Only water is required for the analysis (cost and eco-friendly)

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Analysis of sucrose proposed in USP-NF Pharmacopeial Forum (SC1011-7F)