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Analysis of star polymers using the Agilent 1260 Infinity Multi-Detector GPC/SEC System

2 Jul 2023

In this application note, Agilent demonstrates the use of the Agilent 1260 Infinity Multi-Detector GPC/SEC System for analyzing star polymers. The system employs a light scattering detector to accurately calculate molecular weights, while a viscometer determines the number of arms in the polymers. The system's exceptional stability and sensitivity allow for distinguishing subtle differences in molecular density that arise from varying numbers of arms in the star polymers.

1260 Infinity II Multidetector GPC/SEC System

Agilent Technologies

With the 1260 Infinity II Multidetector GPC/SEC System, you can select from any combination of detection by refractive index, light scattering, and viscometry. The system delivers accurate, absolute molecular weights and sizes, and can be used to analyze a wide range of polymers, regardless of molecular weight range or solvent. Each detector has independent temperature control to ensure that high levels of precision and reproducibility can easily be achieved from one day to the next.

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1260 Infinity II Multidetector GPC/SEC System

Agilent Technologies

With the 1260 Infinity II Multidetector GPC/SEC System, you can select from any combination of detection by refractive index, light scattering, and viscometry. The system delivers accurate, absolute molecular weights and sizes, and can be used to analyze a wide range of polymers, regardless of molecular weight range or solvent. Each detector has independent temperature control to ensure that high levels of precision and reproducibility can easily be achieved from one day to the next.

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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Dynamic Light ScatteringDynamic light scattering is used to measure the size distribution of particles in suspension by analyzing the way light scatters off them. It is commonly used in materials science, nanotechnology, and protein characterization. Browse our peer-reviewed product directory to find the best dynamic light scattering systems, compare products, check reviews, and get pricing directly from manufacturers.PolymersPolymers are large molecules made up of many repeat units. Natural polymers such as DNA and proteins are essential to life, whereas synthetic polymers such as polystyrene and polyethylene are used widely due to their functionality. Typical techniques for analysis include GPC, SEC, DSC, FT-IR and NMR.SECSize exclusion chromatography (SEC) separates molecules based on size, ideal for protein purification, polymer analysis, and biopharma applications. SEC ensures precise characterization of biomolecules for research and industry. Explore peer-reviewed SEC products and compare options for your lab.