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Analysis of RNA transcript levels reveals upregulation of hypoxia markers for pancreatic cancer cells cultured in 3D

Corning Life Sciences
6 Feb 2020

Pancreatic ductal adenocarcinoma (PDAC) is a notoriously aggressive tumor type due to its high levels of metastasis and recurrence and is the fourth leading cause of cancer-related deaths in the Western world. Pancreatic tumors typically lack vasculature and are hypoxic due to oxygen diffusion limitations. In this study, the PDAC cell line PANC-1 was seeded at densities ranging from 500 to 5,000 cells in Corning spheroid microplates and the relative expression of hypoxia markers HIF-1α, GLUT-1, and CA IX were analyzed compared to 2D culture using RT-qPCR to assess the correlation between spheroid size and hypoxia marker expression.

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With their novel and proprietary design, these microplates are ideal for generating and analyzing 3D multicellular spheroids in the same microplate. The Ultra-Low Attachment (ULA) surface enables uniform and reproducible 3D multicellular spheroid formation. The black opaque microplate body shields each optically clear, round bottom well from well-to-well cross-talk.

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Ultra-Low Attachment microplates feature a covalently bound hydrogel layer that effectively inhibits cellular attachment Surface minimizes protein absorption, enzyme activation and cellular activation Surface is noncytotoxic, biologically inert and nondegradable Sterilized by gamma irradiation Black wall microplates have low background fluorescence, minimal light scatter and reduced crosstalk Opaque walls to prevent well-to-well crosstalk Can be used for both top and bottom reading instruments Bulk packed 10 per bag Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body

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Corning Life Sciences

Ultra-low attachment surface with covalently bonded hydrogel that minimizes cell attachment, protein absorption, enzyme activation and cellular activation The surface is noncytotoxic, biologically inert and nondegradable Round bottoms with 300 µL total volume Recommended working volumes of 0.075 to 0.30 mL Sterilized by gamma radiation Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.Cancer ResearchCancer research aims to understand the mechanisms of cancer development and progression to improve prevention, diagnosis, and treatment. From molecular biology to clinical trials, research spans a wide range of disciplines, including immunotherapy, targeted therapies, and drug discovery. Explore the best cancer research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.HypoxiaSLASThe Society for Laboratory Automation and Screening (SLAS) is an organization focused on laboratory automation, high-throughput screening, and biotechnology innovation. SLAS promotes scientific advancements through conferences, publications, and industry collaborations. Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.

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