Analysis of protein crude lysates on the Agilent ProteoAnalyzer System

16 Jun 2025

In this application note, Agilent Technologies assesses the sizing and quantification of protein crude lysates from various tissue types across multiple runs. The consistent results generated in these experiments with the ProteoAnalyzer demonstrate the ability of the system to provide reliable protein measurements.

ProteoAnalyzer System

Agilent Technologies

The Agilent ProteoAnalyzer system is a parallel capillary electrophoresis instrument that automates the capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) analysis of reduced and non-reduced protein samples.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein Extraction
Analysis of protein crude lysates on the Agilent ProteoAnalyzer System