Analysis of oligonucleotides by SEC-MALS

Therapeutic oligonucleotides are produced through a synthetic solid-phase chemical synthesis, which often produces some heterogeneity regarding chain distribution. Monitoring of this distribution is a fundamental aspect of process and quality control, which is typically done by capillary gel electrophoresis (CGE) or anion exchange chromatography. Tosoh Bioscience demonstrates the ability of size exclusion chromatography (SEC) to discriminate oligonucleotides differing by one base in length. Using the TSKgel^® UP-SW2000 size exclusion column, in combination with UHPLC and UHPLC-MALS systems, discover how this column is suitable for oligonucleotide analysis, combined with multi-angle light scattering detection.