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Analysis of Microcystins RR, LY, and YR in Bottled, Tap, and Surface Water Using ACQUITY UPLC Systems with 2D-LC Technology

12 Jan 2015

This application demonstrates the disruptive nature of the ACQUITY UPLC System with 2D-LC Technology with the Xevo TQD Mass Spectrometer. The application targeted the analysis of microcystin RR, LR, and YR in bottled, tap, and surface water. The limit of detection in this study was 50 ppt with a 10:1 enrichment from the extraction protocol (15 min total) and a 200:1 enrichment from the at-column dilution option, for a total of 2000:1.

ACQUITY Systems with 2D Technology

Waters

UPLC Technology Extends To Two Dimensions For chromatographers who require additional capabilities to increase their speed of analysis, gain sensitivity and selectivity, and perform orthogonal separations, ACQUITY UPLC® Systems can meet these needs by controlling multiple valves and pumps for 2D separations. The resolution, sensitivity, and throughput benefits of UPLC® Technology are even more important in 2D applications. Waters’ 2D Technology solutions for the ACQUITY UPLC, ACQUITY UPLC H-Class, and ACQUITY UPLC H-Class Bio systems are purpose-built, from plumbing to software to valve control, to provide reproducible and consistent results for specific applications. Features:   •  Ready-to-use configurations to get you to successful 2D UPLC experiments faster, with less troubleshooting and more confidence   •  ACQUITY UPLC systems and proven columns enable best-in-class selectivity and sensitivity

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Water AnalysisAnalytical methods used to test the purity of drinking water include determining the level of contaminants abides to the level set by the Environmental Protection Agency (EPA).
Analysis of Microcystins RR, LY, and YR in Bottled, Tap, and Surface Water Using ACQUITY UPLC Systems with 2D-LC Technology