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Analysis of Lamotrigine and its Glucuronide Metabolite in Water by Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (LC/Q-TOF-MS) after Automated Solid Phase Extraction
3 Oct 2011This application note describes the conditions necessary to automate the solid phase extraction of lamotrigine and its 2-N-glucuronide metabolite from water samples prior to analysis by LC/Q-TOF-MS using the Gilson GX-271 ASPEC™ System. Extraction recoveries ranged from 75% to 99%. The RSD for inter-day (n=5) values were between 5% and 10%, which showed good reproducibility of the methodology. The LC/Q-TOF-MS limit of detection for lamotrigine and its metabolite were 1 ng/L and 5 ng/L.
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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Environmental Monitoring and TestingEnvironmental monitoring and testing uses handheld portable analyzers, kits, spectrometers or chromatography systems for air, water, soil, food and other sample testing. Useful features of analyzers such as BOD and COD include portability, easy calibration, automation and sensitivity. Environmental test kits for pH, water, moisture, etc, should be accurate, sensitive, reliable, fast and easy to use.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Lamotrigine