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Analysis of Cysteine linked Antibody Drug Conjugates

6 Aug 2020

This Application Note by Agilent demonstrates the DAR determination of brentuximab vedotin using HIC.

Agilent 1260 Infinity III Diode Array Detector WR

Agilent Technologies

The 1260 Infinity III Diode Array Detector WR offers multiple wavelength and full spectral detection up to a 120 Hz sampling rate. Maximum flexibility is guaranteed with a wide wavelength range of 190 to 950 nm with dual-lamp design, as well as the choice of 15 analytical, preparative, and SFC flow cells.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Analysis of Cysteine linked Antibody Drug Conjugates