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Analysis of Cyclodextrines in Green Tea using HPLC-ELSD

16 Aug 2015

Surfactants are well known as a main constituent of commercial detergent with non-ionic surfactants now more widely employed due to their hydrophilic properties meaning they are not ionized even when dissolved in water. Considering its nature, the usage of non-ionic surfactants have increased dramatically and its affect on the environment has been pointed out. Therefore, non-ionic surfactants have been monitored for Water Quality Control Standards. In this application note, Triton X-100 and Polyethylene Glycol 400 as non-ionic surfactant samples were measured and analyzed using an ELSD detector with Silica NH2 column in HILIC mode.

LC-4000 Series Column Ovens

JASCO (USA)

The LC-4000 Series column ovens have been designed to provide excellent utility for a wide range of different user requirements.For simple requirements the stackable CO-4061 can be used with column lengths up to 200mm (or up to 300mm with an optional extension). For RHPLC and UHPLC the CO-4062 has capacity for up to six 150 mm length columns, with optional one or two internally mounted automatic switching valves for 2 or 6 column selection. For GPC and larger preparative columns, select either of the two high-capacity CO- 4060 or CO-4065 ovens. The CO-4060 can accommodate columns with lengths up to 300mm or larger ID preparative columns. The CO-4065 can accommodate up to 10 columns with lengths up to 300mm and a pair of column selection valves is included as standard. All column ovens have built- in electronic cooling for controlling column temperature at sub-ambient temperatures. The CO-4062 and CO-4061 block-heater type ovens can be fitted with an optional micro-volume pre-heating unit for pre- heating the mobile phase before the column to improve resolution and peak shape. The RO-4068 reaction oven is used for high temperature post-column sample processing with capacity for columns and reaction coils.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Environmental Monitoring and TestingEnvironmental monitoring and testing uses handheld portable analyzers, kits, spectrometers or chromatography systems for air, water, soil, food and other sample testing. Useful features of analyzers such as BOD and COD include portability, easy calibration, automation and sensitivity.  Environmental test kits for pH, water, moisture, etc, should be accurate, sensitive, reliable, fast and easy to use.Water Security MonitoringWater AnalysisAnalytical methods used to test the purity of drinking water include determining the level of contaminants abides to the level set by the Environmental Protection Agency (EPA).Environmental ContaminationContaminants in the environment are harmful chemicals that are present in the air, land and water. These include pesticides, pharmaceuticals, persistent organic pollutants (POPs) and many more.Water Content AnalysisWater content analysis is crucial for determining the moisture levels in food, pharmaceuticals, chemicals, and other materials. Accurate measurement ensures product quality and shelf-life. Explore water content analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Environmental AnalysisEnvironmental analysis describes a variety of tests that determine the effect of chemicals, processes and particulates such as persistent organic pollutants (POPs) have on the environment.Beverage AnalysisBeverage analysis involves testing and evaluating the composition, quality, and safety of drinks, from alcoholic beverages to soft drinks. Techniques like chromatography and spectroscopy are commonly used to analyze flavors, nutrients, contaminants, and additives. Browse our peer-reviewed product directory to find the best beverage analysis tools, compare products, check reviews, and get pricing directly from manufacturers.
Analysis of Cyclodextrines in Green Tea using HPLC-ELSD