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Analysis of Common Antioxidants in Edible Oil with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector
4 Nov 2010This note shows that the application of UHPLC to the analysis of common antioxidants in edible oils has resulted in a 53% reduction in run time as well as an 82% reduction in solvent usage per sample. The PerkinElmer Flexar FX-15 UHPLC and Restek® Ultra II C18, 1.9 μm 50 x 2.1 mm column resolved all antioxidants in about three minutes.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.