Analysis of Amino Acids by Automated Pre-column Derivatization with OPA
Analysis of Amino Acids by Automated Pre-column Derivatization with OPA
6 Nov 2015Pre-column derivatization methods for amino acid analysis have been widely used in reversed-phase HPLC, as they offer high selectivity and sensitivity for multiple components. Many derivatization reagents are commercially available and one can choose the most suitable reagent for the application. Among them, orthophtalaldehyde (OPA) is one of the most commonly used reagents because it reacts with amino acids very quickly (seconds) at room temperature and derivatized amino acids can be detected by a fluorescence detector offering increased sensitivity. In this application, the OPA pre-column derivatization is demonstrated by using the automated pre-column derivatization function of JASCO’s autosampler for much better reproducibility than manual sampling.
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LC-4000 Series Autosamplers
JASCO (USA)The LC-4000 Series autosamplers provide a range of options to suit any sample injection requirements, from the AS-4050 entry-level model, to the AS-4250 UHPLC model or the AS-4058 preparative scale autosampler. All LC-4000 series autosamplers share the same simple maintenance features, with consumable parts such as injection ports, rotor seals, and loops accessible from the front panel for easy replacement.