ResourceLife Sciences
Analysis of Akt/mTOR Signalling in Cancer Cell Lines
19 Jun 2011In this application note Dr Joseph Hwang, from EMD Millipore’s Cell Signaling Group, demonstrates the utility of a bead-based multiplex assay that allows for the simultaneous detection of multiple phosphorylated Akt/mTOR pathway proteins. As nearly all of the players in the Akt/mTOR signalling pathway are co-ordinately regulated by phosphorylation, understanding the role of this pathway in normal physiological processes and in diseases such as cancer and diabetes requires the ability to simultaneously measure phosphorylation status of multiple protein targets.
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MILLIPLEX® MAP 11-Plex Akt/mTOR Panel – Phosphoprotein
MerckThe MILLIPLEX® MAP 11-Plex Akt/mTOR Panel – Phosphoprotein is used for the simultaneous quantification of the following Human, Mouse, Rat analytes: Akt (Ser473), GSK3α (Ser21), GSK3β (Ser9), IGF-1R, IR (Tyr1162/Tyr1163), IRS1 (Ser312), mTOR (Ser2448), p70S6K (Thr412), PTEN (Ser380), RPS6 (Ser235/Ser236), TSC2 (Ser939)
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. 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