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Analysis and purification of oligonucleotides by anion exchange and ion-pairing reversed phase chromatography
11 May 2023In this whitepaper, YMC Europe provides useful information for all chromatographers who deal with the analysis and/or purification of oligonucleotides. Therapeutic oligonucleotides (TOs) are synthetic DNA or RNA oligomers designed to target specific genes or transcriptional pathways with the purpose of treating a wide range of diseases, including cancer, genetic disorders, and viral infections. The focus of this whitepaper is on anion exchange chromatography (AEX) and ion pair reversed phase chromatography (IP-RP). For both modes, the main aspects are the selection of a suitable stationary and mobile phase as well as the optimum temperature.
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Polysaccharide HPLC & SFC Columns: CHIRAL ART
YMC EuropeCoated and immobilized CHIRAL ART polysaccharide columns by YMC offer wide selectivity range, high stability and attractive pricing.
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