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Analysis and Purification of Diastereomeric Steroid Saponins from the Trigonella foenum-graecum Seed Extract

Waters
16 Jul 2012

This application note reports the chromatographic resolution of a pair of 25 R/S spirostanol saponin diastereomers from Trigonella foenum-graecum seed extract using the Waters Method Station SFC System. The advantages of using SFC for spirostanol saponin analysis and purification are discussed.

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Analytical Supercritical Fluid Chromatography Method Station II

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Thar Instruments, makers of the TharSFC line of green chromatography Method Station system, is what most people describe as a methods development system for screening multiple compounds with multiple columns and multiple mobile phase compositions. The Analytical Supercritical Fluid Chromatography Method Station II features a smaller footprint despite a new larger Analytical–2–Prep Column Oven and expanded capacity high–speed Alias autosampler and optional Waters Photo Diode Array Detector. Additionally, the new ChromScope software package allows for 21 CFR Part 11 compliant environments as does our Dionex Chromeleon integration.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Supercritical Fluid ChromatographySupercritical Fluid Chromatography (SFC) is used for the rapid separation and analysis of complex mixtures. SFC systems require injectors, pumps, columns, ovens and detectors. The SFC retention method uses a supercritical fluid, usually CO2. There are a number of different SFC detector types including UV / VIs, ECD, mass spectrometry and fluorescence.Steroid SaponinsNatural Products

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Analysis and Purification of Diastereomeric Steroid Saponins from the Trigonella foenum-graecum Seed Extract