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An Enhanced Methodology for Safe, Rapid Freeze Drying of HPLC Fractions

Genevac
2 Oct 2017

Drying high performance liquid chromatography (HPLC) purification fractions, principally comprising water and acetonitrile, is a routine yet essential task in many laboratories. The requirement is to dry the samples to a powder form, such that samples can be accurately weighed, easily sub-sampled and redissolved. Freeze drying is therefore the preferred technology, however, large scale traditional freeze driers may have difficulty processing the organic solvents which can boil out of the samples and damage the vacuum pump. This application note details the implementation of the Lyospeed™ method for fast lyophilization by Actelion Pharmaceuticals.

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Series 3 HT Evaporator

Genevac

The all new Series 3 HT evaporator range from Genevac represents the ultimate in solvent removal technology. The latest touchscreen technology, new high performance vacuum pump, and a sleek ergonomic design makes optimising your evaporation processes effortless. High speed, High throughput evaporation Front opening for easy access. New intuitive touchscreen control system for ultimate ease of use Integral condenser meaning a smaller system footprint Rugged design with clean lines and a modern look All the features and functionality you expect from a Genevac HT evaporator including Dri-Pure anti-bumping, automatic end of run, HCl resistance, Inert Gas Purge, Lyospeed and EXALT controlled crystallisation Available in HT-6 and HT-12 format, Series 3 HT evaporators feature a new 6 place rotor which accepts all existing Genevac HT series holders. Sample formats include microtitre plates, tubes, vials, flasks and SampleGenie -  unique flask and sample holder technology which enables large volume samples to be dried or concentrated directly into the small vial of your choice. Intuitive touchscreen controls enhances monitoring and review of the whole evaporation process. For popular solvent removal protocols pre-set 'Press & Go' methods makes operation easy and productive even for occasional users. Simplified manual and automatic programming means even the most complex multi-stage evaporation methods can be set-up and run quickly and easily. Genevac HT Evaporators are designed to provide the ideal solution for evaporation bottlenecks in the drug discovery laboratory. High performance and high sample capacities make them the ideal workhorses for the busy medicinal chemistry laboratory. Applications: High Throughput Chemistry and Parallel Synthesis LC/MS or HPLC Purification Fast Lyophilisation of purification fractions Natural Products Research Oligosynthesis Flash Chromatography Process Development.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Evaporators and Freeze DryersCentrifugal evaporation is used for solvent removal from a sample. This is a useful technique for sample concentration or sample drying. Many centrifugal evaporators are modular with a central vacuum pump for generating low pressure. Useful features of evaporators or vacuum ovens include minimal solvent bumping, solvent resistance, computer control, temperature regulation and benchtop design.LyophilizationChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.HPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.

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