SelectScience
Join Free
Accelerating Science
ResourceSeparations

An Efficient HPLC Method for Quantitation of Vitamin C in Human Plasma Using Impact™ Protein Precipitation Plate Technology with Kinetex® HPLC Columns

Phenomenex Inc
27 Mar 2015

As the determination of vitamin levels in patients becomes an increasingly important factor in the monitoring and diagnosis of diseases, high-throughput laboratories must adopt a rapid and robust method to accurately analyze and quantitate vitamins and their derivatives. This application note demonstrates a method developed by Phenomenex that can be easily automated, can be used in high-throughput labs, and provides sensitivity, speed, and reproducibility.

Related products

Request Quote for All Products

Impact™ Protein Precipitation Plates

Phenomenex Inc

Impact™ protein precipitation plates with Solvent Shielding Technology™ offer a rapid and convenient way to remove proteins from plasma and tissue homogenate samples prior to analysis.The Solvent Shielding Technology design will withhold organic solvents above the filter membranes for up to 25 minutes, allowing for direct in-well precipitation upon sample addition. The precipitate is then filtered out via vacuum, centrifuge or positive pressure resulting in a clean, protein depleted extract.

(0)

Links

Phenomenex IncCompany website

Tags

UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.Human PlasmaHuman plasma is the liquid component of blood, which carries blood components - nutrients, hormones, proteins, and waste - throughout the body. Plasma-based studies provide insights into biomarkers, immune responses, and disease mechanisms. Compare plasma handling tools and systems in our peer-reviewed product directory.Vitamin C

Related resources

An Efficient HPLC Method for Quantitation of Vitamin C in Human Plasma Using Impact™ Protein Precipitation Plate Technology with Kinetex® HPLC Columns