An Automated Solution to Antibody Purifications

9 Sept 2014

This poster demonstrates the development of high capacity magnetic Protein A and magnetic Protein G beads by combining two novel technologies.

Maxwell® 16 LEV RNA FFPE Purification Kit

Promega Corp.

  The Maxwell® 16 LEV RNA FFPE Purification Kit is designed to extract RNA rapidly from FFPE samples using the Maxwell® 16 Instrument (Cat.# AS2000 and AS3000).   The FFPE kit is specifically designed for optimal purification of RNA from 5μm thin sections of formalin-fixed, paraffin-embedded (FFPE) tissue samples. The Maxwell® 16 LEV RNA FFPE Purification Kit is optimized to yield a final RNA concentration that maximizes RNA yield, eliminating the need to concentrate the extract prior to amplification. Lysates are placed directly into the cartridges, and RNA ready for amplification is obtained in approximately 60 minutes (after proteinase K digest). This kit does not use hazardous xylene, thereby providing a much safer method than many competitive kits. Additionally, quality testing demonstrates virtually no PCR inhibitors in purified samples. Features & Benefits: Automated Platform for Extracting RNA from FFPE Mammalian Tissues: Provides an alternative to manual competitive offerings. Walkaway Automated Extraction from up to 16 Samples in a Single Run: Provides superior ease of use over competition. Provides High Yield, Pure and Amplifiable RNA from FFPE Mammalian Tissue Samples: Minimizes sample waste and reruns. Simple Method with No Organic Solvents for Deparaffinization: No exposure to toxic organic reagents to deparaffinize the FFPE tissue sample prior to RNA purification. Decreased Processing Time: Protocol can be completed within a single 8-hour shift. Applications: Automated RNA purification from FFPE samples.

(0)

Magne™ Protein A and Magne™ Protein G Beads

Promega Corp.

Magne™ Protein G and Magne™ Protein A Beads are magnetic affinity beads with high specificity and high capacity for purification of immunoglobulins from cell culture media, ascites and serum samples.   These paramagnetic beads are composed of iron encapsulated in macroporous cellulose with low nonspecific binding. The magnetic beads use a novel attachment chemistry to immobilize recombinant Protein G or Protein A protein molecules in the same orientation on the surface of the bead. The oriented attachment is known to improve the functionality of immobilized proteins. These beads offer a convenient method for achieving high purity and high recovery of monoclonal and polyclonal antibodies from a variety of biological samples. The superb magnetic properties of Magne™ Protein G and Magne™ Protein A Beads allow rapid and efficient capture of antibodies either with manually processed samples or in a high-throughput manner using the Promega ReliaPrep™ LV 32 HSM Instrument or a robotic platform such as the Beckman Coulter Biomek® FX. Magne™ Protein A and Magne™ Protein G Beads:   High purity and rapid, efficient antibody recovery Low nonspecific binding Ease of handling and cost effectiveness High binding capacity1 (≥25mg) of the beads for Protein A and Protein G Optimized performance

(0)

Links

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Antibody PurificationMagnetic BeadsMonoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.
An Automated Solution to Antibody Purifications